The Rapid Detect nicotine urine test cassette is a lateral flow, one-step immunoassay for the qualitative detection of Nicotine. Cotinine (COT), is an alkaloid found in tobacco and is also a major metabolite of nicotine The word “Cotinine” is an anagram of “nicotine”. Cotinine is used as a biomarker for exposure to tobacco smoke.
Tobacco smoking results in the absorption of nicotine through the lung and buccal/nasal epithelium, after which nicotine is metabolized into about 20 metabolites excreted in urine.
Cotinine, a major metabolite, accumulates in the body with regular smoking. It is reported that it is stable in body fluids and has a relatively long half-life of approximately 17 hours. Therefore, the detection of Cotinine is less dependent on the time of sampling than that of nicotine and other metabolites.
Cotinine levels <10 ng/mL are considered to be consistent with no active smoking. 0 ng/mL to 100 ng/mL are associated with light smoking or moderate. Levels above 300 ng/mL are seen in heavy smokers – more than 20 cigarettes a day.
Cotinine assays provide an objective quantitative measure that is more reliable than smoking histories or counting the number of cigarettes smoked per day.
The iScreen OFD nicotine saliva test for qualitative detection of nicotine in oral fluids. It also permits the measurement of exposure to second-hand smoke
The iScreen OFD, nicotine saliva test method utilizes antibodies to selectively detect elevated levels of nicotine in oral fluid. Saliva testing does not require a bathroom and the procedure can be completed without any difficulty at a desk within an office setting. Both test kits are completely disposable and should be discarded after use. if necessary, make a photocopy displaying your test results.